Effect of 4.25% PD fluid treatment on the peritoneum of MR1+/+ C57BL/6J and MR1-/- C57BL/6J mice

Mr1 knockout (Mr1-/-) mice, in which thymic cells lose the ability to promote the development of mature Mucosal-associated invariant T (MAIT) cells, induced systemic MAIT cell depletion. MAIT cells often exhibit altered functions during fibrosis and inflammatory diseases. Here we used intraperitoneal injections of 4.25% PD fluid (0.1 mL/g) daily for six weeks to mimic peritoneal dialysis (PD) related fibrosis in mice, and investigated whether Mr1 knockout could ameliorated PD-induced changes in peritoneal transcriptome. We analyzed bulk RNA-seq of peritoneums from Mr1+/+ + PBS group (n=3), Mr1+/+ + PD group (n=3), Mr1-/- + PBS group (n=2), and Mr1-/- + PD group (n=2), and demonstrated that Mr1 knockout could ameliorated PD-induced activation in fibrosis, hyperglycolysis, and mTORC1 signalings. In addition, the activation scores of these pathways are positively correlated with each other. Overall design: A cohort of Mr1+/+ and Mr1-/- C57BL/6 male mice received intraperitoneal injection of PBS or 4.25% PD fluid of the same volume for 6 weeks. After modeling, mice were euthanized, and peritoneums were collected for sqeuencing.