Although CRISPR/Cas9 has been widely used to generate knockout mice, two major limitations remain: the founders usually carry a mixture of genotypes, and mosaicism harboring multiple genotypes. Theref
To increase the targeting scope and diversify the editing patterns of base editors, we designed a subset of base editors derived from the Cas9 ortholog from Staphylococcus aureus (SaCas9) in which cyt
We have developed the ACGBEmax system, which is created by fusing a dual-functional deaminase, an engineered N-methylpurine DNA glycosylase, and an evolved SRAP domain with nCas9 (D10A). This system e
