tRF-seq landscapes the variation of tRNA derived fragments after mouse liver injury

In order to intensively elucidate the metabolic characteristics of tRFs after liver injury, we performed tRF-seq by high throughput sequencing. We found that after liver injury, large abundance of tRFs were produced. Especially, the tRF-1s largely vary during the liver injury process and may play important role in the liver disease. Overall design: WT and NS-KO mice were once or repeated injected CCl4, recorded as Day 3 or Day 27. The control mice of WT or NS-KO were injected corn oil, recorded as Day 0. The mice livers were collected and used for tRF-seq, in duplicate, using Illumina NextSeq 500 system.