ISH probes: Primers, amplicon sizes and temperatures used in the hybridization assays.
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Gene-specific forward (F) and reverse (R) primers were used to generate antisense RNA probes for use in the in situ hybridization (ISH) assays. All R primers also contained the T7 RNA promoter sequence (T7PS) at the 5’-end (5´-TAATACGACTCACTATAGGGAGA-3´). The amplicon sizes and temperatures used in the ISH assays are also shown.
创建时间：
2018-04-06
