fungal community of 3-year-old silver fir (soil and fine roots).xlsx

Samples were collected in June 2017 in Poland. Samples SP30, were collected from soil at a nursery in an open field where 3-year-old (3/0 – production symbol used in Polish nurseries) fir seedlings were produced. Samples SP21, were collected from the same location, but from soil under 3-year-old (2/1 – production symbol commonly used in Polish nurseries) fir seedlings that had been transplanted (to reduce their density) after their second year of production. Samples KOST, consisted of peat substrate derived from nursery containers comprising a system based on J. Kosterkiewicz’s method (Robakowski et al., 2021). Samples SO, were collected from soil in a nursery under a Scots pine canopy, and samples SW, were collected from a nursery under a Norway spruce canopy. Fir seedling production methods carried out at studied nurseries are described by Robakowski et al. (2021). Finally, samples NAT, were collected from forest soil in a mature fir stand with natural regeneration. In total, 30 soil samples and 30 fine-root samples from 3-year-old seedlings were harvested. Nursery I, where 3/0 seedlings and 2/1 transplants were produced - WGS 84 N:50.2304, E:16.7280. This location also hosted a nursery that produced fir from peat substrate in nursery containers, following the system proposed by J. Kosterkiewicz. Nursery II included a location under Scots pine shelterwood and natural regeneration in a fir stand (WGS 84: N: 50.2883, E: 16.7001). Nursery III was located under Norway spruce shelterwood (WGS 84: N: 50.2000, E: 16.7540). Fungi levels were identified based on their ITS 1 region and 5.8 S rDNA component. Analysis was conducted using Illumina SBS technology, and the obtained sequences were compared with reference samples deposited in the UNITE database using the BLAST algorithm.