The transcriptomic profile of human microvascular endothelial cells after exposure to preeclamptic or normotensive third trimester trophoblast debris. Homo sapiens

The human placenta is covered by a single multinucleated fetal cell, the syncytiotrophoblast, which is bathed in maternal blood. During all pregnancies, membrane enclosed extracellular vesicles derived from the syncytiotrophoblast are extruded into the maternal blood.The large size of these extracellular vesicles (diameter larger than 10 µm) is referred to as trophoblast debris in this study. We have shown in the past that trophoblast debris from first trimester can contribute to maternal cardiovascular physiological adaptations. This study aimed to characterise the transcriptional changes that occur in human vascular endothelial cells following exposure to preeclmaptic and normotensive trophoblast debris. Primeview human gene expression arrays were used to probe transcriptomic changes in human microvascular endothelial cells after exposure to preeclmaptic or normotensive trophoblast debris for 21 hours Overall design: Trophoblast debris were isolated by four individual preeclmaptic and three gestational controlled normotensive human placentae. HMEC-1 cells were co-cultured with trophoblast debris (60ug/ml total debris protein contents) for 21 hours before RNA extraction. Untreated HMEC-1 cells were used as negative controls. In total, 10 samples were analyzed.