Expression data of ECa9706 over expressing miR-144/451 cluster

miR-144/451 cluster is highly conversed in different species, miRbase database shows miR-144/451 cluster is constituted by miR-144-3p, miR-144-5p, miR-451a. Low-expression of miR-144/451 was closely related with the risk for esophageal cancer We used microarrays to identify the differentially expressed genes in ECa9706 over expressing miR-144/451 cluster To obtain pri-miR-144/451 cluster plasmid, a synthetic sequence within the range of upstream 200bp to downstream 200bp of pri-miR-144/451 was cloned into the PCDH-CMV-MCS-EF1-Puro lentivirus plasmid. Plasmids of pMDLg/Prre, pRSV-Rev, pMD2G and PCDH-CMV-MCS-EF1-Puro were transfected into HEK-293T for packaging lentivirus. We detected the expression of genes in cells overexpressing miR-144/451 cluster (A9547, A9548 and A9549) and the control (A9546, A9547 and A9548).