Data & Code: "Bacterial diversity in the waterholes of the Kruger National Park: an eDNA metabarcoding approach"
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https://figshare.com/articles/dataset/Data_Code_Bacterial_diversity_in_the_waterholes_of_the_Kruger_National_Park_an_eDNA_metabarcoding_approach_/7119668
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资源简介:
Data and code to reproduce:
Farrell, M.J., Govender, D., Hajibabaei, M., van der Bank, M., Davies, T.J. (2018) "Bacterial diversity in the waterholes of the Kruger National Park: an eDNA metabarcoding approach", Genome doi: 10.1139/gen-2018-0064
Scripts1. DADA2 pipeline for quality filtering, denoising, chimera removal, ASV calling, and taxonomy assignment (dada2_pipeline.R)2. Build phylogeny (build_phylo.R) - R script with system calls to QIIME and FastTree3. Community and biodiversity analyses (community_analyses.R)
Required DataThe community analyses can be reproduced with the data files included here. To reproduce the DADA2 pipeline and phylogeny building, the raw sequence reads are archived in the NCBI Sequence Read Archive:
BioProject PRJNA490450Accessuib numbers SRR7822814 to SRR7822901
In addition you will need the DADA2 formatted training fasta files for SILVA version 128 (https://zenodo.org/record/801832#.W5_UPPMbCEI) and the SILVA 128 release for QIIME (https://www.arb-silva.de/download/archive/qiime/)
创建时间:
2018-11-30



