Chronic Prenatal Delta-9-tetrahydrocannabinol Exposure Adversely Impacts DNA methylation in a Rhesus Macaque Model [methylation]

Epigenome-wide profiling of DNAm in samples obtained from 10 rhesus macaques Edibles were administered prior to the animal’s daily chow to ensure consumption on an empty stomach and to confirm complete ingestion. Animals were slowly titrated up to 2.5mg/7kg/day of THC using published weight-based medical cannabis acclimation recommendations approximately 4 months prior to undergoing time-mated breeding. Each THC-exposed pregnant animal (n=5) continued to consume a daily edible of 2.5mg/7kg/day throughout pregnancy. Illumina MethylationEPIC analysis performed on 5 tissues from 10 animals (5 THC; 5 CON). One heart sample (right ventricle) was not collected at necropsy. One Cerebellum sample was removed due to low signal intensity.