Gene expression profile at single cell level of the lung tissue of the septic mice

Single-cell sequencing of mouse lung tissue from the Sham group, CLP group, CLP+Maresin1 and the CLP-48h group. Overall design: We used the Cecal ligation and puncture (CLP), the most commonly used method to induce polymicrobial sepsis, to construct the mouse model of septic lung injury. The mice in Sham group , CLP group,CLP+Maresin1 group were sacrificed 24 hours after operation, while the CLP-48h group were sacrificed at 48 hours after operation.The mice in the CLP+Maresin1 group were administered 1 ng of Maresin1 via the tail vein. The lung tissue samples were collected for subsequent testing and analysis. Three sham group specimens, three CLP specimens, three CLP-48h specimens and three CLP+Maresin1 group specimens were mixed for single-cell RNA sequencing. The prepared single cell suspensions was subjected to Magnetic-activated cell sorting (MACS) using Miltenyi mouse CD45 magnetic beads according to the manufacturer's recommendations. The CD45- and CD45+ cell suspensions were mixed 1:1 for single-cell sequencing.