DamID of Dam::EMR-1 in Caenorhabditis elegans L1 larvae

The execution of developmental programs of gene expression requires an accurate partitioning of the genome into distinct compartments, with heterochromatin enriched at the nuclear periphery. In C. elegans embryonic cells, the methylation of histone H3 lysine 9 (H3K9me) is critical for peripheral anchoring via the chromodomain protein CEC-4, while alternative, H3K9me-independent pathway(s) function in differentiated tissues. An RNAi screen unexpectedly identified the euchromatin factor MRG-1, which binds H3K36me2/me3 domains, as necessary for heterochromatin sequestration in differentiated cells. The data in this submission refer to the identification of chromatin enriched at the nuclear periphery (as measured by interaction with Dam::EMR-1) in intestinal cells of control, singly depleted cec-4 or mrg-1 or doubly depleted cec-4 mrg-1 animals. Overall design: Comparison of Dam::EMR-1 DNA association in wild type, mrg-1(RNAi), cec-4(ok3124) and mrg-1(RNAi) cec-4(ok3124) backgrounds. Strains expressing a nuclear GFP::Dam fusion were used to normalise for changes in chromatin accessibility. Using a Pelt-2::FLP driver to induce genome recombination (Mu oz-Jim nez et al, PMID: 28646043), the Dam fusion proteins were expressed uniquely in intestine. Three biological replicas were prepared: all strains were grown in parallel in each replica. For technical reasons, we eliminated one replica of mrg-1 (RNAi), which was not included in this series.