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Analysis of ENG-modulated gene expression profiles in MPNST cells

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NIAID Data Ecosystem2026-05-01 收录
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In order to study the gene expression changes related to ENG silencing in MPNST cells, we performed RNA-seq gene expression profiling after knockdown of ENG in STS26T cells compared to shScramble control. MPNST cells were transduced with lentiviral particles encoding shRNA designed to silence human ENG (shENG) or a non-targeting control scrambled sequence (shScramble). RNA was extracted with trizol and subsequent Qiagen columns. RNA sequencing was performed afterwards.

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2023-07-21
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