Proteasome machinery is instrumental in a common gain-of-function program of the p53 missense mutants in cancer.. Homo sapiens

Mutant p53 proteins, resulting from the missense mutations of the TP53 tumor suppressor gene, possess gain-of-function activities and are among the most robust oncoproteins in human tumors. They are potentially important therapeutic targets. No studies to date have distinguished common, therapeutically relevant mutant p53 gain-of-function effects from effects specific to different mutant variants and cell backgrounds. here we perform the analysis of transcriptomes,rRegardless of the cell background, of different mutant p53s. Overall design: Four different cell lines were transfected with control or p53 siRNA.So The study comprises four experimental cell lines, each in triplicate.