Targeted Editing of PERVs in specific regions of the Sus scrofa genome

We sequenced two pig fetuses and made targeted edits in specific genome regions to target PERVs (Porcine Endogenous Retroviruses). These edited regions are approximately 5-8Kb in length. The number of intact conserved regions containing PERV copies in the pig population ranges from 12 to 18. Our first goal is to map out the number of PERV copies in our population. Additionally, we aim to screen the reads for expected C-to-T base conversions and the introduction of a "stop" codon. The project has two primary goals: 1) Map the frequency of PERV in F9 and F13 piglet genomes and 2) Screen the expected C to T substitution, which leads to the 'stop' codon from the PacBio HiFi sequence reads.