Metagenomic exploration of Lasundra hot spring

Geothermal areas are the main permanent hot places of the world, and these areas are the major habitats of thermophilic bacteria. In culture independent method, metagenome was isolated using manual and kit based methods. The short gun sequencing of metagenome was performed using Ion torrent PGM platform. The analysis of sequencing data revealed that this spring is dominated by bacterial taxa. MG-RAST based community analysis showed that bacteria were found in dominancy with 99.2% in domain hit distribution. Firmicutes was found to be dominant in domain hit classification followed by proteaobacteria. Moreover, unclassified sequences also found in this metagenome indicate there are possibilities of getting novel organisms.