The effects of Pectobacterium brasiliense 1692 outer membrane vesicles on Phytophthora parasitica motility and metabolism

We employed a phenotypic microarray analysis to investigate the ability of Phytophthora parasitica INRA 310 zoospores treated with Pectobacterium brasiliense 1692 OMVs (referred to as treated zoospores) to utilise various carbon sources provided by Biolog. These were compared to the negative control zoospores that were not treated with Pbr1692 OMVs (referred to as untreated zoospores). We separately added the treated and untreated zoospores into the inoculating fluid based on Table 2.1, aliquoted them into the 96 well microplates containing carbon sources at unknown concentrations, as per the manufacturer’s recommended instructions, and incubated the microplates for 48 hrs 

The datasets also include a motility test  of  P. parasitica INRA 310 zoospores treated with Pbr1692 cells and Pbr1692 OMVs to test their effect on P. parasitica INRA 310 zoospores.  When zoospores were tested with Pbr1692 cells, ddH2O was used as the negative control  and and when zoospores were tested with Pbr1692 OMVs, PBS buffer was used as the used as a negative control. In both the experiments, Pbr1692 cells or OMVs were incubated with P. parasitica INRA 310 zoospore for 3 hours, visualising the motility of the zoospores every 30 minutes. Zoospores treated with Pbr1692 cells showed a reduced motility after 2 hours 30 minutes of the incubation, however, when incubated with Pbr1692 OMVs zoospores motility change was not observed.

本研究采用表型微阵列分析技术，探究经pectobacterium brasiliense 1692 OMVs（以下简称处理后的游动孢子）处理的Phytophthora parasitica INRA 310游动孢子利用Biolog提供的各种碳源的能力。这些游动孢子与未经Pbr1692 OMVs处理的阴性对照游动孢子（以下简称未处理游动孢子）进行了比较。根据表2.1，将处理后的和未处理的游动孢子分别添加到含有未知浓度碳源的96孔微孔板中，按照制造商的推荐操作规程进行，并在48小时内孵育微孔板。此外，数据集还包括对经Pbr1692细胞和Pbr1692 OMVs处理的Phytophthora parasitica INRA 310游动孢子的游动性测试，以评估其对游动孢子的影响。在用Pbr1692细胞进行测试时，ddH2O用作阴性对照；在用Pbr1692 OMVs进行测试时，使用PBS缓冲液作为阴性对照。在两次实验中，Pbr1692细胞或OMVs均与Phytophthora parasitica INRA 310游动孢子共孵育3小时，每隔30分钟观察游动孢子的游动情况。经Pbr1692细胞处理的游动孢子在孵育2小时30分钟后显示出游动能力的下降，然而，与Pbr1692 OMVs孵育的游动孢子游动性未发生明显变化。