The base editing results of BE-PLUS(AID) by WGS

To understand the biology of functional genomic elements over the non-coding genome is considered as one of the next frontiers of genome study. Current available CRISPR-based base editing (BE) provides a sequence preference tool to explore genomic elements at single-nucleotide resolution. By comprehensive bioinformatics analysis, we found “GC” dinucleotides are enriched in regulatory regions including promoter and 5'UTR region. To satisfy the requirements of this genomic elements with various sequence context, we developed a new version of base editor, BE-PLUS(AID) without sequence preference, which performs efficient and precise base editing. Taking advantages of this advanced BE, we successfully manipulated the G rich G4 elements, and explored their regulatory function in gene expression. Taken together, we developed a versatile tool for investigating functional genomic elements.