A summary of the structural changes of the EGL after enzyme treatment.
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IU: International unit; HUVEC: human umbilical vein endothelial cell; BAEC: bovine aortic endothelial cells; CLSM: confocal laser scanning microscopy; WGA: Wheat germ agglutinin; FITC: Fluorescein isothiocyanate; LRB-SC: Lissamine rhodamine B sulfonyl chloride;aUnit: for heprinase III, one IU = 600 Sigma Unit; for hyaluronidase, one IU = one National Formulary unit (Sigma used).bCationic probes: Alcian blue. Lectin: FITC– and LRB-SC–WGA selectively binds to N?acetylglucosamine (HS, HA) and N?acetylneuraminic acid (SA) residues [54], [56]; Biotinylated-B.simplicifolia lectin has the ability to recognize N-acetylglucosamine (CS) residues.cThe structural changes of the EGL. I: Fluorescence intensity; T: Thickness; ALS: Surface area lacking signal.
创建时间:
2015-12-02



