Genome location of derived mutations in the B87 and T50 lines.

Single base mutations identified in haploid spores from the vegetative (3B-87, 4B-87) and meiotic (3T-50, 4T-50) lines and in diploids from the vegetative (2B-52) line. All mutations in the vegetative lines were heterozygous and all mutations in the meiotic lines were homozygous. Single base mutations observed in the B and T lines were annotated relative to the S288C reference genome (Saccharomyces genome data base (SGD); http://www.yeastgenome.org). The SGD coordinate and the amino acid change due to the mutation are shown. Deletion phenotype of the gene, if inviable, is also indicated.