Combining recombinase-mediated cassette exchange strategy with quantitative proteomic and phosphoproteomic analyses to inspect intracellular functions of the tumor supressor galectin-4 in colorectal cancer cells.

In order to systematically explore Galectin-4 function in CRC we established a CRC cell line where Gal4 expression can be regulated via doxycycline (dox)-inducible expression of a single copy wildtype LGALS4 transgene generated by recombinase-mediated cassette exchange (RMCE). Using this model and applying in depth proteomic and phosphoproteomic analyses we systematically screened for intracellular changes induced by Gal4 expression by.