George H. Patterson (2010) CIL:721, Chlorocebus sabaeus. CIL. Dataset

COS 7 cell expressing Organelle LightsTM ER-GFP (Invitrogen) consiting of the signal sequence of the ER protein calreticulin and a KDEL
retention sequence. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.07 µm x 0.07 µm and pixel time was set to 1.28 µsec. Detector pinhole was set to collect 25 ~0.5 µm slices at 0.5 µm intervals.

COS 7 细胞表达 Organelle LightsTM ER-GFP（Invitrogen）载体，该载体包含内质网蛋白钙网蛋白的信号序列及KDEL保留序列。图像采集采用蔡司 LSM510 META（Carl Zeiss, Thornwood, NY）显微镜，配以63倍1.4 NA Plan-Apochromat 油浸物镜，激发波长为488 nm，使用HFT 488 二色滤光片及LP505 发射滤光片。图像分辨率为1024 x 1024，像素大小为0.07 µm x 0.07 µm，像素采集时间设定为1.28 µsec。探测器光阑设置为在0.5 µm间隔下收集25 ~0.5 µm厚的切片。