mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [mRNA]

To study the molecular signatures of mRNAs and miRNAs which can potentially act as a prognostic biomarkers in pancreatobiliary and intestinal types of periampullary adenocarcinomas Total RNA was isolated from 10 x 20 µm sections of 81 fresh frozen tumor samples for mRNA expression profiling. A total of 49 PDAC, 8 bile duct adenocarcinoma, 8 ampulla of pancreatobiliary tye, 7 ampulla of intestinal type, 9 duodenum sample and 12 adjacent normal samples were used. These samples were analysed by microarray analysis using the 60K agilent platform. The expression profiles were background corrected and quantile normalized for mRNA. Filtering of controls, unannotated genes and low expressed probes was done.