COQ4 decarboxylates MHDB

COQ4 is a zinc-dependent enzyme that catalyzes the decarboxylation step in CoQ biosynthesis (Pelosi et al., 2024 and Nicoll et al., 2024). However, the substrate and the product of the decarboxylation step remain ambiguous since Nicoll et al. reported a decarboxylation of 3-methoxy-4-hydroxy-5-decaprenylbenzoate (MHDB) into 2-methoxy-6-decaprenylphenol (DMPhOH), whereas Pelosi et al. proposed that COQ4 catalyzes an oxidative decarboxylation which yields a hydroxylated product. In this case, DHB rather than MHDB was proposed a substrate for COQ4, generating 3-polyprenyl-4-hydroxyphenol as a product. We are provisionally showing the first reaction until more data is available.

COQ4是一种依赖锌的酶，它催化辅酶Q生物合成中的脱羧反应步骤（Pelosi等人，2024年及Nicoll等人，2024年）。然而，由于Nicoll等人报道了3-甲氧基-4-羟基-5-脱氧癸酰苯甲酸（MHDB）的脱羧产物为2-甲氧基-6-脱氧癸酰苯酚（DMPhOH），而Pelosi等人则提出COQ4催化的是氧化脱羧反应，生成羟基化的产物。在这种情况下，DHB而非MHDB被提出作为COQ4的底物，从而生成3-多聚癸酰基-4-羟基苯酚作为产物。鉴于数据尚不充分，我们暂且展示这一初步反应。