Data sets for: Iterative Safe-by-Design Development of an Antibacterial Coating: Balancing Efficacy and Safety for Prosthetic Joint Infection Prevention

An innovative coating combining polycationic poly-ε-lysine (PEL) and hyaluronic acid (HA) previously demonstrated promising in vitro results. However, subsequent in vivo results on antibacterial efficacy were inconsistent. Therefore, this study aimed to enhance the antibacterial efficacy of the coating by increasing PEL concentration twofold (from PEL-10 to PEL-20), elevating HA levels fivefold (from HA-1 to HA-5), and/or adding polyarginine (PAR30-4). These four coating formulations underwent in vitro safety and antibacterial efficacy assessments based on Safe-by-Design (SbD) principles. In vitro testing included bacterial adhesion, osteoblast behavior, and immune response evaluations.