Effects of two classes of helix A mutations on 1a-induced ER membrane rearrangements, BMV RNA3 accumulation, RNA replication, 1a and 2aPol accumulation, and localization.

a(average number of spherules in yeast cells with a clear nucleus in the plane of section among a total of two hundred yeast cells sectioned / average number of spherules in wt 1a expressing yeast cells with a clear nucleus in the plane of section among a total of two hundred yeast cells sectioned) ×100%.b(average number of yeast cells with a clear nucleus and layers in the plane of section among a total of two hundred yeast cells sectioned / average number of wt 1a expressing yeast cells with a clear nucleus and layers in the plane of section among a total of two hundred yeast cells sectioned) ×100%.caverage of independently measured diameters of 50 spherules±standard deviation.d[(RNA3 signal - RNA signal without 1a)/(RNA3 signal with wt 1a – RNA signal without 1a)] ×100%.