WT and N88S seipin-expressing cells upon bathophenanthrolinedisulfonate (BPS) treatment

WT and N88S seipin-expressing cells were grown in SC-glucose medium to exponential phase and then iron chelator bathophenanthrolinedisulfonate (BPS) was added to a final concentration of 100 micromolar. Cells were incubated in the presence of BPS for an additional 4 hour period. We intend to evaluate differentially expressed genes (DEGs) between the two strains. Four biological replicates (classified as 1-4 for the corresponding strain) were used per strain.