Electronic Supplementary Materials

<b>Electronic Supplementary Materials </b>for the article "Comparative performance of bulk and eDNA metabarcoding data in freshwater biomonitoring under the Water Framework Directive".<b>Supplementary Material 1. </b>List of sampling sites and their detailed characteristics processed using DNA metabarcoding in this study. (Supplementary Material 1.xlsx)<b>Supplementary Material 2. </b>List of species (DNA dataset) obtained in this study using the DNA metabarcoding approach after merging replicates in TTT. The dataset includes results for individual sampling sites and sample types, with species presence indicated by read counts and absence marked as 0. (Supplementary Material 2.xlsx)<b>Supplementary Material 3. </b>List of metrics used to calculate the Multimetric Index for ten different stream types. (Supplementary Material 3.xlsx)<b>Supplementary Material 4. </b>Reference values for metrics used in partial EQR (Ecological Quality Ratio) calculations by stream type. (Supplementary Material 4.xlsx)<b>Supplementary Material 5. </b>Graphical representation of the number of detected taxa across different taxonomic levels (species, genus, family, order) for three datasets (OTUs, Linnean species, and ASTERICS) and sample types (bulk, water, sediment). (Supplementary Material 5.tiff)<b>Supplementary Material 6. </b>Values of individual metrics from ASTERICS and EQR calculations for ecological status assessment: individual sheets for each stream type include metric values (A.) and calculated partial EQRs (B.); additional sheets summarize all partial EQRs, final multimetric EQRs, and classification into five ecological status classes. (Supplementary Material 6.xlsx)