Cholecystokinin facilitates motor skill learning by modulating neuroplasticity in the motor cortex

Cholecystokinin (CCK) is an essential modulator for neuroplasticity in sensory and emotional domains. Here, we investigated the role of CCK in motor learning using a single pellet reaching task in mice. Mice with a knockout of cck gene (CCK-/-) or blockade of CCK-B receptor (CCKBR) showed defective motor learning ability; the success rate of retrieving reward remained at the baseline level compared to the wildtype mice with significantly increased success rate. We observed no long-term potentiation (LTP) upon high-frequency stimulation (HFS) in the motor cortex of CCK-/- mice, indicating a possible association between motor learning deficiency and neuronal plasticity in the motor cortex. In vivo calcium imaging demonstrated that the deficiency of CCK signalling disrupted the refinement of population neuronal activity in the motor cortex during motor skill training. Anatomical tracing revealed direct projections from CCK-expressing neurons in the rhinal cortex to the motor cortex. Inacti..., , , \#Title: Cholecystokinin facilitates motor skill learning by modulating neuroplasticity in the motor cortex

\[doi:10.5061/dryad.9ghx3ffms]

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Success rate WT-CKO.xlsx: Success rate of WT (wildtype) and CCK-/- (cholecystokinin gene knockout) mice. W represents wildtype mice and KO represents CCK (cholecystokinin) gene knockout mice. Every single mouse was identified with different serial numbers, like W-1, W-2, KO-1,KO-2, and so on (Figure. 1C and Figure 1-figure supplement 1);

WT-CKO hausdorff distance.xlsx: Hausdorff distance of trajectories from WT and CCK-/- mice at Day 1 and Day 3. C57 represents wildtype mice and CCK-/- represents CCK gene knockout mice. (Figure 1E);

Detail performance_WT_CKO.xlsx: Detailed performance of WT and CCK-/-...