Metadatafile.csv

Approximately 7-10 fecal pellets from individual mice were collected prior to terminal analyses at week 52 post-therapy. Fecal pellets were aseptically collected and stored at -20 oC. Samples were shipped to Utah State University where sample processing and microbiota studies were performed<strong>. </strong>Briefly, isolated total community DNA was isolated from the fecal samples using a QIAGen QIAamp DNA stool mini kit according to the instructions provided by the manufacturer. Upon obtaining total DNAs, samples were sent to Idaho State University (ISU) Molecular Core Facility for Illumina MiSeq next generation sequencing (https://www.isu.edu/research/centers-and-institutes/molecular-research-core-facility/services/). Briefly, first stage PCR was performed to amplify the V3-V4 region of 16S rRNA from total DNA. Subsequently, the PCR products for each sample were cleaned using Ampure XP beads. Then, a second stage PCR was performed for Illumina indexing. Upon running Illumina MiSeq for the samples, the raw fastaq files were hosted at the site managed by Idaho State University and their sequencing facility to perform the bioinformatics analysis using Mothur software package and we received processed data files. These files were uploaded to microbiomeanalyst.ca for selected analyses using Mothur output files and SILVA taxonomy as the reference 16S rDNA database.