beta 2 Adrenergic receptor expression analysis by flow cytometry on CD4+ T cells isolated from mPGES-1 KO or WT mouse splenocytes

Flow cytometric data from five experiments analyzing beta-2-adrenergic receptor expression on CD4+ T cells (MACS positive selection from spleen) from mPGES-1 ko (-/-) or WT (+/+) mice subjected to vagus nerve stimulation (VNS) and intraperitoneal LPS injection 30 min prior to sample collection.

Panel (more detailed info can be found in the "FCS file identification list" document):
FITC-Viability
PE-b2AR
PCPCy5.5-CD69
PECy7-CD62L
APC-CD44
APCCy7-CD3
BV421-CD25
BV510-CD4

The protocol for staining, cell extraction VNS etc. is described in the publication "Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mpuse spleen" published in PLOS ONE 2017 by the authors: Priya Revathikumar, Johanna Estelius, Utsa Karmakar, Erwan LeMaître, Marina Korotkova, Per-Johan Jakobsson and Jon Lampa.