Evolutionary isolation and ecological stasis in the endangered Comal springs Dryopid beetle (Stygoparnus comalensis)

The Comal Springs dryopid beetle (Stygoparnus comalensis) is an endangered, subterranean-obligate, long-toed water beetle known only from three spring complexes in the Edwards Aquifer region of Texas. Due to their small size and reliance on groundwater karst habitats, little is known of the biology of these beetles.
The aim of this study was to investigate evolutionary, ecological and morphological divergence among known S. comalensis localities in order to inform conservation management plans for this species.
We used genotyping-by-sequencing (n = 85), stable isotope ($\delta^{15}$N and $\delta^{13}$C) analysis (n = 104), and morphometric analysis (n = 80), to describe variation among the three known localities and to ask whether ecological or morphological differentiation is associated with population genomic divergence. 
S. comalensis populations are highly structured with no evidence of gene flow. Stable isotope ratios and morphology did not significantly differ among populatio..., , # Evolutionary isolation and ecological stasis in the endangered Comal springs Dryopid beetle (Stygoparnus comalensis)

Dataset DOI: [10.5061/dryad.qrfj6q5t6](10.5061/dryad.qrfj6q5t6)

## Description of the data and file structure

Files included here contain morphological, stable isotope, and population genomics data for Stygoparnus comalensis, the blind dryopid beetle.

Dryopid_isotopes.csv contains the stable isotope data (dC13 and dN15) for all sampled individuals (n = 104). The first column codes are: ca = Comal Springs Adult, cl = Comal Springs larva, fa = Fern Bank Springs adult, fl = Fern Bank Springs larva, sa = Sessom Springs adult.

stygoparnus_morph_dryad.csv contains the morphometric data (body length, body width). Column \"complex\" is the spring complex (c = Comal Springs, f = Fern Bank Springs, s = Sessom Springs). Column \"SNPs\" identifies individuals that also have SNP genotypes (y/n).

gprobmean.csv is the matrix of posterior genotype estimates for 85 salamanders for 52...,