Data from: Population growth of native and invasive strains of the larger grain borer, Prostephanus truncatus on three maize hybrids

Insects
Two different populations of Prostephanus truncatus were used in the bioassays, one originated from the invaded range in Ghana, and the other from the native range in Mexico. Both populations were maintained in the Laboratory of Entomology and Agricultural Zoology (LEAZ), at the Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Greece, on whole maize kernels, at 26°C and 55% relative humidity (RH) and continuous darkness.
European Maize Hybrids
Three different maize hybrids (“PICO”, “HAMILTON”, and “AGN 672”) were obtained from American Genetics SA, Sindos, Greece. All hybrids were cultivated at Serres, in northern Greece according to the local farming practices. The hybrid “PICO” has a great production potential and it is adapted to multiple soil types and can produce high-weight grain. The hybrid “HAMILTON” is a dual-purpose hybrid, that has excellent early vigor and it is tolerant to fungi, and the hybrid “AGN 672” has excellent early vigor and it is also tolerant to fungi.
Population Growth on Different Maize Hybrids
Three different maize hybrids (PICO, Hamilton, and AGN 672) were used for experimentation. These hybrids were untreated and uninfested, and kept at ambient conditions until the beginning of the experiments. Before proceeding with the bioassays, grain moisture content (M.C.) was assessed, using a moisture meter (mini-GAC plus, Dickey-John Europe S.A.S., Colombes, France). Standardized plastic vials as in prior work (Quellhorst et al. 2023; Lampiri et al. 2022) were used here (3 cm in diameter, 8 cm in height). Vials were then filled with 20 g of one of the three maize hybrids with lids added after. The commodity was weighed with a Precisa XB3200D compact balance (Alpha Analytical Instruments, Gerakas, Greece). The upper rings of the vials were treated with Fluon (Northern Products Inc., Woonsocket, USA) to prevent insects from moving away from the grain and/or escaping. The top of each vial also had small holes punched to allow ventilation. Each vial then received 10 P. truncatus adults of mixed sex and age from one of two different strains. Two different populations of P. truncatus were used as mentioned above. The vials were placed inside incubators set at 30°C and 65% R.H. in continuous darkness. The vials were removed from the incubators after 45 d and adult progeny production was recorded. We also recorded the weight of frass, the number of insect-damaged kernels (IDK), and the total weight of the kernels within each vial. For each combination, i.e. hybrid × strain, there were n = 9 replicates.

在生物试验中，采用了两种不同群体的 Prostephanus truncatus，一种源自加纳的入侵范围，另一种源自墨西哥的本土范围。这两种群体均由希腊塞萨洛尼基农业大学农业、作物生产与乡村环境系昆虫与农业昆虫学实验室（LEAZ）进行维护，培养在完整的大麦籽粒上，温度为26°C，相对湿度（RH）为55%，并在持续黑暗的环境中饲养。欧洲玉米杂交品种从希腊辛多的美国遗传学SA公司获得的三种不同的玉米杂交品种（“PICO”、“HAMILTON”和“AGN 672”）。所有杂交品种均按照当地农业习惯在希腊北部的塞雷斯进行栽培。杂交品种“PICO”具有巨大的生产潜力，适应多种土壤类型，并能产出高重量的谷物。杂交品种“HAMILTON”是一种双用途杂交品种，具有出色的早期生长活力，对真菌具有耐受性，而杂交品种“AGN 672”也具有出色的早期生长活力，同样对真菌具有耐受性。在不同玉米杂交品种上的种群增长：在实验中使用了三种不同的玉米杂交品种（PICO、Hamilton和AGN 672）。这些杂交品种未经处理且未受侵害，在环境条件下保持直至实验开始。在生物试验之前，使用湿度计（mini-GAC plus，Dickey-John Europe S.A.S.，法国科尔贝）对谷物水分含量（M.C.）进行了评估。与先前研究（Quellhorst等人，2023年；Lampiri等人，2022年）中使用的标准塑料试管一样，这里也使用了（直径3厘米，高度8厘米）的试管。然后在试管中装入20克三种玉米杂交品种之一，并添加盖子。商品使用Precisa XB3200D紧凑型天平（Alpha Analytical Instruments，希腊Gerakas）称重。试管的顶部环用Fluon（Northern Products Inc.，美国伍斯特克特）处理，以防止昆虫远离谷物或逃逸。每个试管的顶部也打有小型孔洞，以允许通风。每个试管接收来自两个不同品系的10只混合性别和年龄的P. truncatus成虫。正如上述提到的，使用了两种不同的P. truncatus群体。试管放置在设置在30°C和65% R.H.的连续黑暗中的孵化器中。在45天后，从孵化器中取出试管，记录了成虫后代的生产量。我们还记录了粪球的重量、虫害粒数（IDK）以及每个试管中谷物的总重量。对于每种组合，即杂交品种×品系，有n = 9个重复。