Increased rate of de novo single nucleotide variants in house mice born through assisted reproduction

This project investigates whether assisted reproductive technologies (ART), such as in vitro fertilization, may affect the genetic integrity of offspring. Using a well-controlled mouse model, we compared genetic mutation rates between mice conceived through ART and those conceived naturally. By analyzing whole-genome sequencing data, we aimed to understand whether ART procedures are associated with an increased number of new (de novo) genetic mutations. This work provides insight into the potential genetic consequences of ART and helps inform future research on the safety and long-term impacts of reproductive technologies. We obtained and provided two independent sequence libraries (seq1 and seq2) for all the samples in this pedigree. For more information regarding each sequencing run, please refer to the methods.