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ORBIT for E. coli - mutant library sequencing

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NIAID Data Ecosystem2026-05-01 收录
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Oligo Recombineering followed by Bxb-1 Integrase Targeting (ORBIT) is a method for reverse genetics in E. coli. Hundreds of deletion mutants were constructed simultaneously using oligo pools and the quality of these mutant libraries was assessed with Illumina sequencing.

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2023-06-29
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