Genomic profiles of oxidative stress-induced rat renal cell carcinomas

Intraperitoneal administration of ferric nitrilotriacetate (Fe-NTA) initiates Fenton reaction in the renal proximal tubules of rodents that ultimately leads to a high incidence of renal cell carcinoma (RCC) after repeated treatment. We performed high-resolution microarray comparative genomic hybridization to identify characteristics in the genomic profiles of oxidative stress-induced rat RCCs. The results revealed extensive large-scale genomic alterations with a preference for deletion.向啮齿类动物腹腔给予次氮基三乙酸铁（ferric nitrilotriacetate, Fe-NTA），可在其肾近端小管中触发芬顿反应（Fenton reaction）；经反复给药处理后，最终会导致动物出现极高的肾细胞癌（renal cell carcinoma, RCC）发病率。本研究通过高分辨率微阵列比较基因组杂交（high-resolution microarray comparative genomic hybridization）技术，对氧化应激诱导的大鼠肾细胞癌的基因组谱特征进行鉴定。结果显示，此类肿瘤存在广泛的大规模基因组变异，且以染色体缺失为主要偏好类型。