Transcriptome analyses of tumor-infiltrating dendritic cells (TIDC) from WT versus Tsc22d3 conditional knockout (Tsc22d3-cKO) mice under stress and chemotherapy conditions

In this context (Stress and MTX-based chemotherapy of established tumors), TIDC were purified from WT and Tsc22d3-cKO mice, followed by RNAseq-based transcriptome analyses . The absence of Tsc22d3 favored the upregulation of IFN-ß stimulated genes in TIDC , indicating that Tsc22d3 acts to repress the type I IFN response, which is known to be required for anticancer immunosurveillance. Interestingly, the SD-inhibited expression of several genes involved in MHC I-restricted antigen presentation was partially reversed (Calr, Sec61b, Sec61g) in Tsc22d3-deficient TIDC . However, the expression of TLRs, antigen uptake and cell adhesion molecules, chemokines/cytokines and receptors (except Ifnb1 and Ccl5) remained largely unchanged in TIDC lacking Tsc22d3, compared with their WT counterparts.