Expression data (HG-U133 Plus 2.0) from human monocyte-derived cell types (TolDC, M-MDSC, MoDC)

A better understanding of the mechanisms that induce and drive human suppressive myeloid cells, such as myeloid-derived suppressor cells (MDSCs) and tolerogenic dendritic cells (TolDCs), stimulates therapeutic approaches that intent to restore the immune balance in patients. Here, we compared gene profiles of in vitro generated M-MDSCs to TolDCs in the context of monocytes and monocyte-derived DCs (MoDCs). Human M-MDSCs, TolDCs and MoDCs were generated in vitro from CD14+ purified Monocytes for 5 donors. Four cell types from 5 donors were used for RNA extraction and hybridization on Affymetrix microarrays. Three Monocyte samples failed QC leaving a total of 17 samples in this analysis.