MoTrPAC: Endurance Exercise Training Study In 6 Months Old Rats

MoTrPAC endurance exercise training study targets young adult (6-month-old) rats. Specifically, male and female Fischer 344 rats (n = 12) were subjected to progressive treadmill endurance exercise training for 1, 2, 4, or 8 weeks, with tissues collected 48 hours after the last exercise bout. Sex-matched sedentary, untrained rats were used as controls. This study contributes to MoTrPAC's broader mission, which is to identify molecular changes occurring due to exercise. The ultimate goal is to understand how physical activity can improve and sustain health across various ages and fitness levels. The program is supported by the NIH Common Fund. For additional information, visit the MoTrPAC Data Hub at https://motrpac-data.org/. This SuperSeries is composed of the SubSeries listed below. 6-month-old young adult rats (Fischer 344) were subjected to progressive endurance exercise training for 1, 2, 4, or 8 weeks, with tissues collected 48 hours after the last training bout. Sex-matched sedentary, untrained rats were used as controls. Whole blood, plasma, and 19 solid tissues were analyzed using genomics, proteomics, metabolomics, and protein immunoassay technologies, with most assays performed in a subset of these tissues. Depending on the assay, between 3 and 6 replicates per sex per time point were analyzed.