small RNA sequencing analysis of TRMT1L KO vs WT brain

TRMT1L is a paralog of the tRNA m22G-methyltransferase TRMT1 and, similarly to TRMT1, it is involved in cognitive functioning. To functionally dissect the biological role of TRMT1L, here we have employed a combined strategy of LC-MS/MS and next-generation sequencing of both long and short RNAs, to compare the levels and locations of m2,2G in brain tissues from TRMT1L knock-out mice, relative to wild-type. small RNASeq for 2 conditions (KO and WT), with biological triplicates.TRMT1L是tRNA m²²G-甲基转移酶TRMT1的旁系同源基因（paralog），且与TRMT1类似，其参与认知功能的调控。为解析TRMT1L的生物学功能，本研究采用液相色谱-串联质谱（LC-MS/MS）结合长、短RNA下一代测序的联合策略，对比TRMT1L基因敲除（KO）小鼠与野生型（WT）小鼠脑组织中m²,2G的表达水平及分布位置。本研究针对两种实验条件（KO组与WT组）开展小RNA测序，每组设置生物学三重重复。