Endoderm differentiation of human embryonic stem cells (hESCs) [RNA-Seq]

Purpose: The goal of this study is to identify the whole-genome transcriptome profiling of differentially expressed genes during human embryonic stem cell differentiation. Methods: Triplicate RNA profiles were generated from hESCs and induced endoderm cells, by deep sequencing with Illumina NextSeq 500. Results: We identified 6320 genes differentially expressed between hESCs and induced endoderm cells, with foldchange  1.5 and P < 0.05. Conclusion: Our study confirms that endodermal gene were dramatically induced during endoderm differentiation.

研究目的：本研究旨在开展人类胚胎干细胞分化过程中差异表达基因的全基因组转录组谱分析。
实验方法：以人类胚胎干细胞（human embryonic stem cells, hESCs）与诱导型内胚层细胞为材料，采用Illumina NextSeq 500平台进行深度测序，生成了三份生物学重复的RNA转录组谱。
研究结果：本研究鉴定出人类胚胎干细胞与诱导型内胚层细胞之间共存在6320个差异表达基因，其表达倍数变化≥1.5且P值<0.05。
研究结论：本研究证实，在内胚层分化过程中，内胚层相关基因的表达被显著上调。

搜集汇总

背景与挑战

背景概述

该数据集是一个人类胚胎干细胞向内胚层分化的RNA-Seq研究，包含6个样本（3个干细胞和3个诱导内胚层细胞），使用Illumina NextSeq 500平台进行测序，总数据量为7.49 GB。研究识别出6320个差异表达基因，证实了内胚层基因在分化过程中的显著诱导，为干细胞分化机制提供了转录组层面的关键见解。