RNA sequencing, TAB- and TAK1-dependent transcriptomic alternations in response to lysosomal damage

Lysosomes are the primary degradative organelles and play a pivotal role in maintaining cellular and tissue homeostasis. Lysosomal dysfunction is associated with various diseases, underscoring the crucial role of lysosomal integrity and its quality control in human health Lysosomes are susceptible to damage by many sources, both internal and external to the cell, which results in the permeabilization and rupture of lysosomal membranes. Crystals, amyloids, pathogens, lipids, lysosomal toxic drugs, and reactive oxygen species (ROS) are among the stressors that can damage lysosomal membranes. To gain insight into the involvement of the TAB-TAK1 pathway in the regulation of gene expression associated with the lysosomal damage response, we performed transcriptome analysis of TAB- and TAK1-depleted RPE-1 cells treated with or without L-Leucyl-L-Leucine methyl ester (LLOMe), a well-characterized inducer of lysosomal damage, for 2 h.