Table_9_Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes.xlsx

The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development.

Nepeta tenuifolia叶片上的盾形腺毛（PGTs）能够分泌并储存生物活性精油。单细胞RNA测序（ScRNA-seq）是一种揭示异质性细胞并探究特定细胞发育与分化的强大工具。鉴于叶片富含PGTs，研究人员选取幼叶以分离原生质体，并成功捕获了33,254个原生质体用于测序。经过细胞类型注释后，所有细胞被划分为六个广泛的群体，共19个簇。基于腺毛特异性基因、单萜生物合成基因的表达模式以及PGT分泌物的代谢分析，鉴定了来自PGTs的细胞。通过拟时间分析，描绘了PGTs的发育轨迹。对N. tenuifolia叶片与拟南芥（Arabidopsis thaliana）芽的scRNA-seq数据进行整合分析，揭示了PGTs在N. tenuifolia中具有特异性。因此，我们的研究结果为探索植物细胞发育与分化提供了有前景的基础，尤其是在腺毛的起始和发育过程中。