RNA-seq Density Tracks of Primary Human Kidney Glomerular Outgrowths, Tubular Cultures and Immortalized Podocytes

Kidney tissues were obtained from patients undergoing radical nephrectomy for renal tumors with informed consent for DNA sequencing (University of Washington IRB 1297). Normal kidney cortex was lightly digested and then mechanically sieved to isolated glomeruli. The glomeruli were allowed to attach and primary outgrowth cultures of podocytes and mesangial cells were obtained in ~14 days. In parallel, cortical tissue was used to generate tubular outgrowth cultures. These cultures and an immortalized human podocyte cell line were subjected to DNase-seq and RNA-seq per published ENCODE protocols.