Effects of canagliflozin on SGLT1-, SGLT2-, and facilitative glucose transporter-mediated glucose transport, and on SGLT3-induced currents.

CHOK cells over-expressed with human, rat, or mouse SGLT1 or SGLT2 and rat L6 myoblast cells were used. AMG or 2-DG uptake was determined and IC50 values were calculated as described in Methods section. Data of human SGLT1 and SGLT2 were presented the summary of 5 studies. Other data presented the mean value of 2 experiments. Oocytes expressed with human SGLT3 were used to determine canagliflozin effect on DNJ-induced current.2-DG, 2-deoxy-d-glucose; AMG, alpha-methylglucoside; CHOK, Chinese hamster ovary-K; IC50, concentration required to inhibit 50% of activity; SGLT, sodium glucose co-transporter; DNJ, imino sugars 1-deoxynojirimycin.