Investigating the effects of estrogen in the transcriptional regulation of SOX2 in breast cancer cells.

We have evaluated the effects of estrogen over SOX2 expression by maintaining MCF-7 under three distinct conditions: estrogen deprivation for a year (LT -E2 MCF-7), treating MCF-7 cells with estrogen for 48 hours (48hr +E2 MCF-7), and maintaining cells under estrogenic conditions for a year (LT +E2 MCF-7). Overall design: LT -E2 MCF-7 cells were maintained in estrogen-deprived conditions for a year; 48hr +E2 MCF-7 cells were treated with 10nM b-estradiol for 48 hours; LT -E2 MCF-7 cells were grown under 10nM b-estradiol for a year. Cells were submitted for ATAC-seq and ribo-depleted total RNA-seq in three replicates each.