Advanced 4-chamber echocardiography techniques enable clinically matched precise characterization of heart disease progression in mice

Background: Transthoracic echocardiography remains the primary non-invasive method for assessing cardiac function in clinical practice. However, technical challenges in acquiring accurate apical 4-chamber-long-axis (A4CLAX) views have historically limited mouse studies to left ventricle (LV) assessment using parasternal short-axis (SAX) M-mode imaging.
Methods: To overcome this limitation, we developed a novel A4CLAX imaging approach for mice and performed a comparative analysis with established echocardiographic methods to assess cardiac function in healthy mouse hearts. To evaluate the utility of A4CLAX in detecting disease progression, we longitudinally monitored cardiac function of C57BL/6N mice (male and female) following severe transverse aortic constriction (TAC), using both long-axis biplane (LAX-BP) and conventional SAX M-mode assays.
Results: Here we show that LAX-BP echocardiography demonstrates volumetric accuracy comparable to cardiac magnetic resonance (CMR) and detects si..., , # Advanced 4-chamber echocardiography techniques enable clinically matched precise characterization of heart disease progression in mice

Dataset DOI: [10.5061/dryad.wdbrv161r](10.5061/dryad.wdbrv161r)

## Description of the data and file structure

A zipped files contains all quantification echocardiographic assessment in mouse hearts.

### Files and variables

#### File: COMMSMED-24-0778A_Supplementary_Data.zip

**Description:** 

The fold includes seven files.

1.    **File: Figure 2-Adult WT mice** **(Prism).** A Prism file contains the data of cardiac echocardiographic assessment in normal mice using long-axis biplane (LAX-BP), parasternal M-mode (M-mode), short-axis Simpson’s biplane (SAX-BP), parasternal long-axis (PLAX), and apical 4-chamber long-axis (A4CLAX) approaches. Left ventricle function parameters evaluated include heart rate (HR), end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF), stroke volume (SV), and cardiac output (CO).

2.    **File: F...,