Observed rate constants (kobs, s-1) of SNARE complex formation measured by fluorescence anisotropy (VAMP2 labeled) for Sx4 constructs in the presence and absence of Munc18c.

Values are shown as mean kobs ± s.d. from three independent experiments. Bold text indicates the observed rate constant (kobs) for Sx41-275 SNARE assembly in the absence and presence of Munc18c showing that SNARE assembly is 7-fold slower in the presence of Munc18c for this construct only. Shading indicates all four experiments for which Munc18c was included, showing that Munc18c did not inhibit SNARE assembly when the Sx4 C-terminus had a T4L fusion, or when the Sx4 N-peptide was removed.