Effect of markerless deletion of ptsP from P. aeruginosa PA14 biofilm colonies on M6301-glycerol agar for 3 d at 25C

To determine the transcriptomic effect when ptsP, which encodes enzyme I of the nitrogen-related phosphotransfer system (Nitro-PTS), is absent from cells. Deletion of this gene results in lower exopolysaccharide levels (a biofilm defect). Here, we compared PA14 deleted for amrZ (which has a moderately hyper-biofilm phenotype) and cells deleted for both amrZ and ptsP. We also analyzed wild-type PA14 cells (see GSE226104). Overall design: Total RNA was extracted from mechanically dispersed colonies grown on M6301-1% agar for 3 d at 25C. Transcriptomes were compared among PA14, PA14 delta-amrZ (raw data for these samples are deposited under GSE226104), and PA14 delta-amrZ delta-ptsP. GSE226104/PRJNA938508 re-analyzed samples: GSM7063834 PA14 delta-amrZ cells, biol rep 1 SRX19499901 SAMN33439943 GSM7063835 PA14 delta-amrZ cells, biol rep 2 SRX19499902 SAMN33439942 GSM7063836 PA14 delta-amrZ cells, biol rep 3 SRX19499903 SAMN33439941 GSM7063837 PA14 delta-amrZ cells, biol rep 4 SRX19499904 SAMN33439940