Gene expression profile in mouse back skin after cyclophosphamide treatment

Chemotherapy-induced hair loss (alopecia; CIA) is a highly distressing adverse effect that many cancer patients endure. Yet the molecular pathobiology of CIA remains largely unknown. We analysis the changes of mRNA profiles at different time points after cyclophosphamide treatment using Next-generation Sequencing in a mouse model of CIA. Our result revealed that Shh signaling is an evolutionarily conserved key target in CIA pathobiology. Overall design: All mice used in this study were male C57BL/6 purchased from Shanghai SLAC animal facility center, and housed in Fuzhou University Animal Facility Center. Active hair growth (anagen) was induced in the back skin by wax-depilation in 7-8 weeks old male mice. Cyclophosphamide (CYP, Sigma) was dissolved in PBS and i.p. injected at day 9 post-depilation at a dose of 150mg/kg.Mouse back skin samples were taken before (T0) or at 6hr, 12hr, 24hr, and 48hr after CYP treatment. Total RNAs were isolated using Trizol reagent (Invitrogen). Total RNAs were quality monitored by Agilent 2100 analysis, and sequenced by Illumina Genome Analyzer in Novogene Corporation., Beijing, China.