Analysis of the stability and functionality of ONC variants.
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aTd were determined in 0.1 M MES-NaOH buffer, pH 6.0 by DSC as described in Material and Methods.bIC50 (±SD) values were determined using the CellTiter96® cell viability assay as described in Material and Methods.cUnits of catalytic efficiency, kcat/KM, are 102 M−1s−1. Values of kcat/KM were determined for catalysis of 6-FAM-dArUdAdA-6-TAMRA cleavage at 25°C in 0.1 M MES-NaOH buffer, pH 6.0, containing 0.1 M NaCl [43].
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2015-12-02
